pDONR223-MEKDD
(Plasmid #31202)

• Depositing Lab: David Root
• Publication: Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638

Backbone

• Vector backbone: pDONR223
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5005
• Vector type: Gateway Donor vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MAPKK1
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• Mutation: S218D S222D
• Entrez Gene: MAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13pUC-fwd
• 3′ sequencing primer: M13rev

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.

A set of 4 custom lentiviral vectors is available (http://www.addgene.org/25895, http://www.addgene.org/25896, http://www.addgene.org/25897, http://www.addgene.org/25890) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.