pEVOL-pAzF
(Plasmid #31186)

• Purpose: tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG
• Depositing Lab: Peter Schultz
• Publication: Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7.

Backbone

• Vector backbone: p15A
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Any strain for growth, 37 oC, LB/2XYT media
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: M.j. p-azidohenylalanine RS (2 copies +tRNA)
• Alt name: pEVOL-pAz
• Species: M.jannaschii
• Insert Size (bp): 1000
• Mutation: Y32T, E107N, D158P, I159L, L162Q, D286R

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: ATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTT

Resource Information

• Addgene Notes:
  • Additional Addgene Diagnostic digest
• Articles Citing this Plasmid:
  • 43 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry