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Addgene

FRB-NLuc
(Plasmid #31181)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31181 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-V5/HIS TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5523
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rapamycin-binding domain and the N-terminus of Firefly Luciferase
  • Alt name
    FRB
  • Alt name
    FLUC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1608
  • Entrez Gene
    MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRB-NLuc was a gift from David Piwnica-Worms (Addgene plasmid # 31181 ; http://n2t.net/addgene:31181 ; RRID:Addgene_31181)

  • For your References section:

    Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals. Luker KE, Smith MC, Luker GD, Gammon ST, Piwnica-Worms H, Piwnica-Worms D. Proc Natl Acad Sci U S A. 2004 Aug 17. 101(33):12288-93. 10.1073/pnas.0404041101 PubMed 15284440
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