pET24c(+)-EFTu
(Plasmid
#31172)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31172 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET24c(+)
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsBL21
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameE. coli elongation factor Tu
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Alt nameEF-Tu
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SpeciesE. coli
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Insert Size (bp)1182
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Entrez GenetufB (a.k.a. b3980, ECK3971, kirT, pulT)
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Tag
/ Fusion Protein
- His (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer vector
- 3′ sequencing primer vector (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET24c(+)-EFTu was a gift from Linda Spremulli (Addgene plasmid # 31172 ; http://n2t.net/addgene:31172 ; RRID:Addgene_31172) -
For your References section:
Role of the conserved aspartate and phenylalanine residues in prokaryotic and mitochondrial elongation factor Ts in guanine nucleotide exchange. Zhang Y, Li X, Spremulli LL. FEBS Lett. 1996 Aug 12. 391(3):330-2. 10.1016/0014-5793(96)00789-2 PubMed 8765000