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Addgene

782 pMEActMEK
(Plasmid #31166)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31166 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pME
  • Backbone size w/o insert (bp) 2503
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MEK
  • Species
    H. sapiens (human)
  • Mutation
    Ser218Glu Ser222Asp; activated MEKK
  • Entrez Gene
    MAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Gateway (not destroyed)
  • 3′ cloning site Gateway (not destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer M13rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Middle entry clone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    782 pMEActMEK was a gift from Nathan Lawson (Addgene plasmid # 31166 ; http://n2t.net/addgene:31166 ; RRID:Addgene_31166)

  • For your References section:

    A genetic screen for vascular mutants in zebrafish reveals dynamic roles for Vegf/Plcg1 signaling during artery development. Covassin LD, Siekmann AF, Kacergis MC, Laver E, Moore JC, Villefranc JA, Weinstein BM, Lawson ND. Dev Biol. 2009 May 15. 329(2):212-26. 10.1016/j.ydbio.2009.02.031 PubMed 19269286
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