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Addgene

474 p5E-basprom
(Plasmid #31158)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31158 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p5E
  • Backbone size w/o insert (bp) 2810
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    basal adenovirus E1b TATA element
  • Alt name
    transcriptional start site from carp b-actin gene

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Gateway (not destroyed)
  • 3′ cloning site Gateway (not destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer M13rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

"p5E" refers to 5' entry clones flanked by L4-R1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    474 p5E-basprom was a gift from Nathan Lawson (Addgene plasmid # 31158 ; http://n2t.net/addgene:31158 ; RRID:Addgene_31158)
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