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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31130 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFLP recombinase
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Alt nameFLP
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1370
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCSFLPe was a gift from Gerhart Ryffel (Addgene plasmid # 31130 ; http://n2t.net/addgene:31130 ; RRID:Addgene_31130) -
For your References section:
FLP and Cre recombinase function in Xenopus embryos. Werdien D, Peiler G, Ryffel GU. Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3. 10.1093/nar/29.11.e53 PubMed 11376165