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Addgene

pCSFLPe
(Plasmid #31130)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31130 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLP recombinase
  • Alt name
    FLP
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1370

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSFLPe was a gift from Gerhart Ryffel (Addgene plasmid # 31130 ; http://n2t.net/addgene:31130 ; RRID:Addgene_31130)
  • For your References section:

    FLP and Cre recombinase function in Xenopus embryos. Werdien D, Peiler G, Ryffel GU. Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3. 10.1093/nar/29.11.e53 PubMed 11376165