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Addgene

pSuper-TatSF1 AB
(Plasmid #31119)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31119 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSuper
  • Backbone manufacturer
    Oligoengine
  • Backbone size w/o insert (bp) 3176
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TatSF1 shRNA
  • Alt name
    TatSF1
  • gRNA/shRNA sequence
    GAGTCAGATGACAAGGAAG
  • Species
    H. sapiens (human)
  • Entrez Gene
    HTATSF1 (a.k.a. TAT-SF1, TATSF1, dJ196E23.2)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

shRNA TatSF1 Target: GAGTCAGATGACAAGGAAG

NB: This shRNA for TatSF1 and Plasmid #31120 both work very well.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSuper-TatSF1 AB was a gift from Mariano Garcia-Blanco (Addgene plasmid # 31119 ; http://n2t.net/addgene:31119 ; RRID:Addgene_31119)

  • For your References section:

    Tat-SF1 is not required for Tat transactivation but does regulate the relative levels of unspliced and spliced HIV-1 RNAs. Miller HB, Saunders KO, Tomaras GD, Garcia-Blanco MA. PLoS One. 2009 . 4(5):e5710. 10.1371/journal.pone.0005710 PubMed 19479034
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