pLenti6-MK1-EHMT2-V5
(Plasmid #31113)

• Depositing Lab: Bernard Futscher
• Publication: Full Length EHMT2-V5 tagged_Bernard W. Futscher (unpublished)

Backbone

• Vector backbone: pLenti6/V5-D-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6963
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: euchromatic histone-lysine N-methyltransferase 2
• Alt name: EHMT2
• Alt name: G9a
• Species: H. sapiens (human)
• Insert Size (bp): 3633
• Mutation: Stop codon mutated to Ala
• GenBank ID: NM_006709
• Entrez Gene: EHMT2 (a.k.a. BAT8, C6orf30, G9A, GAT8, KMT1C, NG36)
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ cloning site: TOPO (unknown if destroyed)
• 3′ cloning site: TOPO (unknown if destroyed)
• 5′ sequencing primer: CMV
• 3′ sequencing primer: V5

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Open Biosystem (Mammalian Genome Collection) Accession # BC018718.1
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Spektor TM, Rice JC. Identification and characterization of posttranslational modification-specific binding proteins in vivo by mammalian tethered catalysis. Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):14808-13. Epub 2009 Aug 18.