OPN(-1206)-luc
(Plasmid #31106)

• Depositing Lab: Gerhart Ryffel
• Publication: Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(3):179-90.

Backbone

• Vector backbone: pGL3-Basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: osteopontin promoter
• Alt name: SPP1
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Mutation: promoter region -1206 to +87
• GenBank ID: NM_000582
• Entrez Gene: SPP1 (a.k.a. BNSP, BSPI, ETA-1, OPN)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

promoter luciferase reporter

Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.