Skip to main content
Addgene

FR_luc
(Plasmid #31098)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31098 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5150
  • Vector type
    Mammalian Expression ; Flp/FRT
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    firefly luciferase
  • Species
    firefly luciferase from pGL3-Basic
  • Insert Size (bp)
    1650

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamH (destroyed during cloning)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

for Flp mediated site directed integration, tetracycline inducibilty, to measure tetracycline regulation by Tet repressor

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FR_luc was a gift from Gerhart Ryffel (Addgene plasmid # 31098 ; http://n2t.net/addgene:31098 ; RRID:Addgene_31098)

  • For your References section:

    Pattern of genes influenced by conditional expression of the transcription factors HNF6, HNF4alpha and HNF1beta in a pancreatic beta-cell line. Thomas H, Senkel S, Erdmann S, Arndt T, Turan G, Klein-Hitpass L, Ryffel GU. Nucleic Acids Res. 2004 . 32(19):e150. 10.1093/nar/gnh144 PubMed 15520459
Related items:
Commonly requested with: