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P2_DD-HNF4A8_C106R
(Plasmid #31096)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31096 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5150
  • Vector type
    Mammalian Expression ; Flp/FRT
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HNF4A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1350
  • Mutation
    splice variant 8 derived from promoter P2 with zinc finger mutation C106R
  • GenBank ID
    NM_175914
  • Entrez Gene
    HNF4A (a.k.a. FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF-14, TCF14)
  • Tags / Fusion Proteins
    • FKBP L106P (N terminal on insert)
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

for Flp mediated site directed integration, tetracycline inducibilty by Tet operator in P2 promoter of HNF4A, shield inducibility by destabilizing domain FKBP L106P

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    P2_DD-HNF4A8_C106R was a gift from Gerhart Ryffel (Addgene plasmid # 31096 ; http://n2t.net/addgene:31096 ; RRID:Addgene_31096)

  • For your References section:

    Improved conditional expression systems resulting in physiological level of HNF4alpha expression confirm HNF4alpha induced apoptosis in the pancreatic beta-cell line INS-1. Senkel S, Waldner C, Ryffel GU, Thomas H. BMC Res Notes. 2009 . 2():210. 10.1186/1756-0500-2-210 PubMed 19835622
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