CMV-218_HNF4A2
(Plasmid #31090)

• Depositing Lab: Gerhart Ryffel
• Publication: Senkel et al BMC Res Notes. 2009 . 2():210.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5150
• Vector type: Mammalian Expression ; Flp/FRT
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HNF4A
• Species: H. sapiens (human)
• Insert Size (bp): 1400
• Mutation: splice variant 2 of HNF4A, deletion of CMV promoter to -218.
• Entrez Gene: HNF4A (a.k.a. FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF-14, TCF14)
• Tag / Fusion Protein:
  • myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

for Flp mediated site directed integration, tetracycline inducibilty by Tet operator in CMV promoter shortened to -218.
Corresponding wild type (full length) CMV promoter construct in FR_HNF4A2 (Thomas et al 2004).