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Addgene

pET21c(+)-mtLeuRS
(Plasmid #31079)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31079 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-21c(+)
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21 (DE3) pArg
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mature form human mitochondrial leucyl-tRNA synthetase
  • Alt name
    LeuRS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2646
  • Mutation
    Includes coding sequence for the mature form. Nt 63-2709. Amino acids 22- 903
  • Entrez Gene
    LARS2 (a.k.a. HLASA, LEURS, PRLTS4, mtLeuRS)
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21c(+)-mtLeuRS was a gift from Linda Spremulli (Addgene plasmid # 31079 ; http://n2t.net/addgene:31079 ; RRID:Addgene_31079)

  • For your References section:

    Expression and characterization of the human mitochondrial leucyl-tRNA synthetase. Bullard JM, Cai YC, Spremulli LL. Biochim Biophys Acta. 2000 Feb 29. 1490(3):245-58. 10.1016/S0167-4781(99)00240-7 PubMed 10684970
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