pcDNA6.2-GW/EmGFP-miR-SUMO23
(Plasmid #31073)

• Depositing Lab: Wulf Paschen
• Publication: Yang et al Biochem Biophys Res Commun. 2009 Apr 24. 382(1):215-8.

Backbone

• Vector backbone: pcDNA6.2-GW/EmGFP
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5699
• Vector type: Mammalian Expression, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SUMO2/3 microRNA
• Alt name: SUMO2/3
• gRNA/shRNA sequence: SUMO2: GATCTGCCTCATTGACAAAC; SUMO3: AATCGAATCTGCCTCATTGAC
• Species: H. sapiens (human)
• Entrez Gene: SUMO2 (a.k.a. HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A)
• Entrez Gene: SUMO3 (a.k.a. SMT3A, SMT3H1, SUMO-3, Smt3B)
• Tag / Fusion Protein:
  • EmGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: attB1 (unknown if destroyed)
• 3′ cloning site: attB2 (unknown if destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry