beta2-spectrin-HA
(Plasmid #31070)

• Depositing Lab: Vann Bennett
• Publication: Bennett Lab Plasmids (unpublished)

Backbone

• Vector backbone: Modified pEGFP-N1 (see note)
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: beta2-spectrin
• Alt name: SptbN1
• Alt name: SPTB2
• Species: H. sapiens (human)
• Entrez Gene: SPTBN1 (a.k.a. DDISBA, ELF, HEL102, SPTB2, betaSpII)
• Tag / Fusion Protein:
  • HA (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-Fwd

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This construct DOES NOT contain EGFP- the EGFP has been removed from the pEGFP-N1 backbone.

The following "mutations" have been located in this construct. The Bennett lab believes they are due to incorrect sequence data originally entered into NCBI; however, please be aware of these in case you are designing primers or doing mutagenesis:

Derivations from published sequence:

2.59 c-->t (silent)
4.23 c-->g (H-->D)
5.99 g-->c (R-->T)
6.3 g-->a (silent)

Please refer to the Addgene NGS result for the fully validated sequence.