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Addgene

Neurofascin-186-HA
(Plasmid #31061)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31061 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Modified pEGFP-N1 (see note)
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neurofascin
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Nfasc (a.k.a. NF)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-Fwd
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct DOES NOT contain EGFP- the EGFP has been removed from the pEGFP-N1 backbone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Neurofascin-186-HA was a gift from Vann Bennett (Addgene plasmid # 31061 ; http://n2t.net/addgene:31061 ; RRID:Addgene_31061)

  • For your References section:

    Structural requirements for association of neurofascin with ankyrin. Zhang X, Davis JQ, Carpenter S, Bennett V. J Biol Chem. 1998 Nov 13. 273(46):30785-94. 10.1074/jbc.273.46.30785 PubMed 9804856

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