AnkyrinB-2XHA
(Plasmid #31057)

• Depositing Lab: Vann Bennett
• Publication: Ayalon et al J Biol Chem. 2011 Mar 4. 286(9):7370-8.

Backbone

• Vector backbone: Modified pEGFP-N1 (see note)
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ankyrin B
• Alt name: ankyrin 2
• Species: H. sapiens (human)
• Entrez Gene: ANK2 (a.k.a. ANK-2, CFAP87, FAP87, LQT4, brank-2)
• Tag / Fusion Protein:
  • 2XHA (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-Fwd

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HA-ankyrin-B was prepared based on an ankyrin-B-GFP template in a pEGFP-N1 vector (Mohler et al., 2002). GFP was cut out between the PmeI and the NotI sites and replaced with a 2XHA-tag encoding sequence (YPYDVPDYA) with a PmeI site on its 5′ end and a stop codon followed by a NotI site on the 3′ end that was synthesized by PCR.