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FXYD2-luc
(Plasmid #31056)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31056 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FXYD2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2381
  • Mutation
    FXYD2 promoter from -2382 to -1
  • GenBank ID
    AF316896
  • Entrez Gene
    FXYD2 (a.k.a. HOMG2, ATP1G1, MGC12372)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FXYD2-luc was a gift from Gerhart Ryffel (Addgene plasmid # 31056 ; http://n2t.net/addgene:31056 ; RRID:Addgene_31056)

  • For your References section:

    HNF1B mutations associate with hypomagnesemia and renal magnesium wasting. Adalat S, Woolf AS, Johnstone KA, Wirsing A, Harries LW, Long DA, Hennekam RC, Ledermann SE, Rees L, van't Hoff W, Marks SD, Trompeter RS, Tullus K, Winyard PJ, Cansick J, Mushtaq I, Dhillon HK, Bingham C, Edghill EL, Shroff R, Stanescu H, Ryffel GU, Ellard S, Bockenhauer D. J Am Soc Nephrol. 2009 May . 20(5):1123-31. 10.1681/ASN.2008060633 PubMed 19389850
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