pET24c(+)-mtEFTu
(Plasmid #30535)

• Depositing Lab: Linda Spremulli
• Publication: Woriax et al Biochim Biophys Acta. 1995 Dec 27. 1264(3):347-56.

Backbone

• Vector backbone: pET24c(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21 for expression. DH5 alpha for plasmid preparations. the Kan resistance is on the cloning vehicle
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mature bovine mitochondrial elongation factor Tu
• Species: B. taurus (bovine)
• Insert Size (bp): 1227
• Mutation: Mature form amino acids 44-452. Mito import sequence not included
• GenBank ID: BOS_22843
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry