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pEGFP-Bax1-2/L-6
(Plasmid #30533)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30533 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    C3-EGFP
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BCL2-associated X protein
  • Alt name
    Bax
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    579
  • Mutation
    F30C E44C C62S L63C C126S P130C
  • GenBank ID
    NP_620116
  • Entrez Gene
    BAX (a.k.a. BCL2L4)
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-Bax1-2/L-6 was a gift from Richard Youle (Addgene plasmid # 30533 ; http://n2t.net/addgene:30533 ; RRID:Addgene_30533)

  • For your References section:

    Bcl-x(L) Retrotranslocates Bax from the Mitochondria into the Cytosol. Edlich F, Banerjee S, Suzuki M, Cleland MM, Arnoult D, Wang C, Neutzner A, Tjandra N, Youle RJ. Cell. 2011 Apr 1. 145(1):104-16. 10.1016/j.cell.2011.02.034 PubMed 21458670
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