pBSHSP:FLP;CMV:tdTomato-SceI
(Plasmid #30523)

• Depositing Lab: Gerhart Ryffel
• Publication: Roose et al Transgenic Res. 2009 Aug . 18(4):595-605.

Backbone

• Vector backbone: I-SceI-pBSII-SK+
• Backbone manufacturer: Genbank DQ836146
• Backbone size w/o insert (bp): 3007
• Vector type: Flp/FRT, heat shock inducible, I-SceI Megabase vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FLP recombinase tdTomato
• Species: S. cerevisiae (budding yeast); synthetic
• Insert Size (bp): 4400
• Mutation: R415H
• GenBank ID: AY678269

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

FLP driven by Xenopus laevis heat shock 70 (hsp 70) promoter with CMV driven tdTomato, both in I-SceI megabase vector

Please note that there are some discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor, including a H415R mutation in the downstream dimer component of tdTomato. This mutation may impact tdTomato expression.