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Addgene

pSUPER retro puro Scr shRNA
(Plasmid #30520)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30520 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUPER retro puro
  • Backbone manufacturer
    OligoEngine
  • Backbone size w/o insert (bp) 6343
  • Modifications to backbone
    Linearized BlgII/HindIII
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Scr shRNA
  • Alt name
    scrambled shRNA
  • Insert Size (bp)
    61

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CAGCGGGGCTGCTAAAGCGCATGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Scramble shRNA: 5’-GCGAAAGATGATAAGCTAA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUPER retro puro Scr shRNA was a gift from John Gurdon (Addgene plasmid # 30520 ; http://n2t.net/addgene:30520 ; RRID:Addgene_30520)

  • For your References section:

    Histone variant macroH2A confers resistance to nuclear reprogramming. Pasque V, Gillich A, Garrett N, Gurdon JB. EMBO J. 2011 May 6. ():. 10.1038/emboj.2011.144 PubMed 21552206
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