Skip to main content
Addgene

pSUPER retro puro macroH2A1 shRNA
(Plasmid #30517)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30517 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSUPER retro puro
  • Backbone manufacturer
    OligoEngine
  • Backbone size w/o insert (bp) 6343
  • Modifications to backbone
    Linearized BlgII/HindIII
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mH2A1 shRNA
  • Alt name
    macroH2A1 shRNA
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    61
  • GenBank ID
    macroH2A1 all 4 isoforms

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GGTCGAACGAGGAGGTTCAAGGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

H2A1 shRNA: 5’-GGGTGCAGACAAATGTGAA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUPER retro puro macroH2A1 shRNA was a gift from John Gurdon (Addgene plasmid # 30517 ; http://n2t.net/addgene:30517 ; RRID:Addgene_30517)

  • For your References section:

    Histone variant macroH2A confers resistance to nuclear reprogramming. Pasque V, Gillich A, Garrett N, Gurdon JB. EMBO J. 2011 May 6. ():. 10.1038/emboj.2011.144 PubMed 21552206
Related items:
Commonly requested with: