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Addgene

pCS2+ Bmi1-cherry-HA
(Plasmid #30516)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30516 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+ att-cherry-HA (C-term cherry-HA)
  • Backbone size w/o insert (bp) 4918
  • Vector type
    Mammalian Expression ; Xenopus/Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bmi1
  • Species
    M. musculus (mouse)
  • Mutation
    NO STOP
  • GenBank ID
    NM_007552.4
  • Entrez Gene
    Bmi1 (a.k.a. Bmi-1, Pcgf4)
  • Tags / Fusion Proteins
    • cherry (C terminal on backbone)
    • triple HA (C terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ Bmi1-cherry-HA was a gift from John Gurdon (Addgene plasmid # 30516 ; http://n2t.net/addgene:30516 ; RRID:Addgene_30516)

  • For your References section:

    Histone variant macroH2A confers resistance to nuclear reprogramming. Pasque V, Gillich A, Garrett N, Gurdon JB. EMBO J. 2011 May 6. ():. 10.1038/emboj.2011.144 PubMed 21552206
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