pCS2+ macroH2A1.2-GFP-HA
(Plasmid #30515)

• Depositing Lab: John Gurdon
• Publication: Pasque et al EMBO J. 2011 May 6. ():.

Backbone

• Vector backbone: pDEST pCS2+ att-eGFP-HA (C term eGFP-HA)
• Vector type: Mammalian Expression ; Xenopus/Zebrafish expression.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: macroH2A1.2-C
• Alt name: macroH2A1.2 isoform 1 ATG NO STOP
• Species: M. musculus (mouse)
• Insert Size (bp): 1119
• Mutation: NO STOP
• GenBank ID: CCDS26557.1
• Entrez Gene: Macroh2a1 (a.k.a. H2AF12M, H2afy, mH2a1)
• Tags / Fusion Proteins:
  • eGFP (C terminal on backbone)
  • triple HA (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry