pENTR macroH2A1.2-C
(Plasmid
#30512)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 30512 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepENTR D TOPO
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2580
-
Modifications to backbonenone
-
Vector typeGateway - donor vector
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Top10
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemacroH2A1.2-C
-
Alt namemacroH2A1.2 isoform 1 ATG NO STOP
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1119
-
MutationNO STOP
-
GenBank IDCCDS26557.1
-
Entrez GeneMacroh2a1 (a.k.a. H2AF12M, H2afy, mH2a1)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 F
- 3′ sequencing primer M13 R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pENTR macroH2A1.2-C was a gift from John Gurdon (Addgene plasmid # 30512 ; http://n2t.net/addgene:30512 ; RRID:Addgene_30512) -
For your References section:
Histone variant macroH2A confers resistance to nuclear reprogramming. Pasque V, Gillich A, Garrett N, Gurdon JB. EMBO J. 2011 May 6. ():. 10.1038/emboj.2011.144 PubMed 21552206
