pENTR Bmi1-C
(Plasmid
#30511)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 30511 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR D TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2580
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Modifications to backbonenone
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Vector typeGateway - donor vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBmi1
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SpeciesM. musculus (mouse)
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MutationNO STOP
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GenBank IDNM_007552.4
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Entrez GeneBmi1 (a.k.a. Bmi-1, Pcgf4)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 F
- 3′ sequencing primer M13 R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR Bmi1-C was a gift from John Gurdon (Addgene plasmid # 30511 ; http://n2t.net/addgene:30511 ; RRID:Addgene_30511) -
For your References section:
Histone variant macroH2A confers resistance to nuclear reprogramming. Pasque V, Gillich A, Garrett N, Gurdon JB. EMBO J. 2011 May 6. ():. 10.1038/emboj.2011.144 PubMed 21552206