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Ex1(-117/+3)
(Plasmid #30493)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30493 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lhx1 promoter
  • Alt name
    lim1 promoter
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    703
  • Mutation
    HNF1 binding site deleted

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutated HNF1 binding site in lhx1 promoter: TcgAgGGTTTAC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ex1(-117/+3) was a gift from Gerhart Ryffel (Addgene plasmid # 30493 ; http://n2t.net/addgene:30493 ; RRID:Addgene_30493)

  • For your References section:

    The nephrogenic potential of the transcription factors osr1, osr2, hnf1b, lhx1 and pax8 assessed in Xenopus animal caps. Drews C, Senkel S, Ryffel GU. BMC Dev Biol. 2011 . 11():5. 10.1186/1471-213X-11-5 PubMed 21281489
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