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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 30483 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHDAC6
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SpeciesH. sapiens (human)
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MutationCatalytically inactive mutant (H216/611A)
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Entrez GeneHDAC6 (a.k.a. CPBHM, HD6, JM21, KDAC6, PPP1R90)
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-HDAC6.DC-FLAG was a gift from Tso-Pang Yao (Addgene plasmid # 30483 ; http://n2t.net/addgene:30483 ; RRID:Addgene_30483) -
For your References section:
The deacetylase HDAC6 regulates aggresome formation and cell viability in response to misfolded protein stress. Kawaguchi Y, Kovacs JJ, McLaurin A, Vance JM, Ito A, Yao TP. Cell. 2003 Dec 12. 115(6):727-38. 10.1016/S0092-8674(03)00939-5 PubMed 14675537
