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Addgene

Magoh198delG
(Plasmid #30481)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30481 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Sport6
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Magoh
  • Alt name
    Mago-nashi homolog
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1000
  • Mutation
    198delG (point nucleotide deletion)
  • Entrez Gene
    Magoh (a.k.a. Mago-m, Mos2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer Sp6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Open Biosystems

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

AluI digestion generates 341 bp, 189 bp, 112 bp. Wt only generates 341 bp +300 bp fragments.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Magoh198delG was a gift from William Pavan & Debra Silver (Addgene plasmid # 30481 ; http://n2t.net/addgene:30481 ; RRID:Addgene_30481)

  • For your References section:

    The exon junction complex component Magoh controls brain size by regulating neural stem cell division. Silver DL, Watkins-Chow DE, Schreck KC, Pierfelice TJ, Larson DM, Burnetti AJ, Liaw HJ, Myung K, Walsh CA, Gaiano N, Pavan WJ. Nat Neurosci. 2010 May . 13(5):551-8. 10.1038/nn.2527 PubMed 20364144
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