pRRL.CMVenh.gp91.EF1aIntron.eGFP
(Plasmid #30469)

• Depositing Lab: Didier Trono
• Publication: Barde et al Gene Ther. 2011 May 5. ():.

Backbone

• Vector backbone: pRRL
• Backbone size w/o insert (bp): 6800
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CMVenh/gp91.EF1alntron.eGFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13-rev
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

From the paper (note that some of these features may not be in this plasmid): All vectors were based on the pRRL-cPPT-hPGK-mcs-WPRE vector, derived from the pRRL-cPPT-hPGKeGFP-WPRE construct. The gp91promoter is the natural myeloid-specific promoter. The CMVenh comes from the peGFP-N1(Clontech, Saint-Germain-en-Laye, France),
the synthetic intron from the pMG5 and the EF1a intron from the pWPI vector http://www.addgene.org/12254 The myeloid-specific enhancer SP107 and SP146 were provided by Senlin Li (Department of Medicine, UTHSCSA, San Antonio, TX, USA). The cHS4 insulator corresponds to the chicken beta-globin 5’-DNase I-hypersensitive
site and was provided by Patrick Salmon (Department of Neuroscience,
Faculty of Medicine, University of Geneva, Switzerland).