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Addgene

(-6kb/-58)mtH4-GFP
(Plasmid #30463)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30463 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSGFP2
  • Backbone size w/o insert (bp) 3812
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HNF1a promoter
  • Alt name
    LFB1 promoter
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    5896
  • Mutation
    Mutation of HNF4 binding site at 5471-5494 (Holewa et al 1996)
  • GenBank ID
    AY471617

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct is derived from plasmid (−6 kb/−58)-GFP [Addgene plasmid number 30460] and contains the mutated HNF4 binding site mut1 as described in B Holewa et al., Mech. Dev. 54 (1996), pp. 45–57

Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    (-6kb/-58)mtH4-GFP was a gift from Gerhart Ryffel (Addgene plasmid # 30463 ; http://n2t.net/addgene:30463 ; RRID:Addgene_30463)

  • For your References section:

    Distinct promoter elements mediate endodermal and mesodermal expression of the HNF1alpha promoter in transgenic Xenopus. Ryffel GU, Lingott A. Mech Dev. 2000 Jan . 90(1):65-75. 10.1016/S0925-4773(99)00230-0 PubMed 10585563
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