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Addgene

(-6kb/-207)-GFP
(Plasmid #30462)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30462 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSGFP2
  • Backbone size w/o insert (bp) 3812
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HNF1a promoter
  • Alt name
    LFB1 promoter
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    5700
  • GenBank ID
    AY471617

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The GFP variant in the plasmid backbone, pCSGFP2, contains the F64L and the S65T mutations resulting in 35 times brighter green emission (as described in Ikawa M et al., Curr. Top. Dev. Biol. 44 (1999), pp. 1–20)

This construct is derived from the (−6 kb/−58)-GFP construct [Addgene plasmid number 30460] by replacing the EcoRI–HindIII fragment (−494 to −58) with the corresponding EcoRI–HindIII fragment (−494 to −207) of the 3Δ207 clone (as described in Zapp D et al., Mol. Cell. Biol. 13 (1993), pp. 6416–6426).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    (-6kb/-207)-GFP was a gift from Gerhart Ryffel (Addgene plasmid # 30462 ; http://n2t.net/addgene:30462 ; RRID:Addgene_30462)

  • For your References section:

    Distinct promoter elements mediate endodermal and mesodermal expression of the HNF1alpha promoter in transgenic Xenopus. Ryffel GU, Lingott A. Mech Dev. 2000 Jan . 90(1):65-75. 10.1016/S0925-4773(99)00230-0 PubMed 10585563
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