pVZmSin3A
(Plasmid
#30455)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 30455 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepVZ-1
- Backbone size w/o insert (bp) 3237
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSin3A
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SpeciesM. musculus (mouse)
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Entrez GeneSin3a (a.k.a. Sin3, mKIAA4126, mSin3A)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nar or NotI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
mSin3A can be released with Nar or NotI on the 5' end and XbaI on the 3'end.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVZmSin3A was a gift from Bob Eisenman (Addgene plasmid # 30455 ; http://n2t.net/addgene:30455 ; RRID:Addgene_30455) -
For your References section:
Mad-Max transcriptional repression is mediated by ternary complex formation with mammalian homologs of yeast repressor Sin3. Ayer DE, Lawrence QA, Eisenman RN. Cell 1995 Mar 10;80(5):767-76. 10.1016/0092-8674(95)90355-0 PubMed 7889570