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Addgene

pCS2+MT-mSin3A
(Plasmid #30452)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30452 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+MT
  • Backbone size w/o insert (bp) 4354
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sin3A
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Sin3a (a.k.a. Sin3, mKIAA4126, mSin3A)
  • Tag / Fusion Protein
    • 6XMyc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+MT-mSin3A was a gift from Bob Eisenman (Addgene plasmid # 30452 ; http://n2t.net/addgene:30452 ; RRID:Addgene_30452)

  • For your References section:

    The Smad transcriptional corepressor TGIF recruits mSin3. Wotton D, Knoepfler PS, Laherty CD, Eisenman RN, Massagué J. Cell Growth Differ. 2001 Sep . 12(9):457-63. PubMed 11571228
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