SS13 lac-inducible GFP reporter circuit
(Plasmid
#30336)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 30336 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSB1A2
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Backbone manufacturerTom Knight, MIT Parts Registry
- Backbone size w/o insert (bp) 2079
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Lac-Promoter (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byMIT Parts Registry
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains the wt lac promoter.
Please note that this plasmid, SS13, was not used in the associated publication (Sleight et al J Biol Eng. 2010). SS24 was used in the experiments described in this paper.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SS13 lac-inducible GFP reporter circuit was a gift from Herbert Sauro (Addgene plasmid # 30336 ; http://n2t.net/addgene:30336 ; RRID:Addgene_30336) -
For your References section:
Designing and engineering evolutionary robust genetic circuits. Sleight SC, Bartley BA, Lieviant JA, Sauro HM. J Biol Eng. 2010 Nov 1;4:12 10.1186/1754-1611-4-12 PubMed 21040586
