w+attB
(Plasmid #30326)

• Depositing Lab: Jeff Sekelsky
• Publication: Sekelsky lab Drosophila phiC31 cloning plasmids (unpublished)

Backbone

• Vector backbone: pUC
• Backbone size w/o insert (bp): 2881
• Vector type: Drosophila transgenesis

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: phiC31 attB, hsp70::w
• Species: D. melanogaster (fly)
• Insert Size (bp): 4414

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (destroyed during cloning)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: M13 -21
• 3′ sequencing primer: M13 reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pW8 (Klemenz, 1987, Nucleic Acids Res. 15: 3947)
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

w[+] is hsp70 basal promoter; gives full w+ (red eyes), without heat shock.