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Addgene

pDEST-attB
(Plasmid #30325)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30325 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST
  • Backbone size w/o insert (bp) 4670
  • Vector type
    Drosophila transgenesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Must be grown in ccdB survival cells.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phiC31 attB site
  • Species
    phage phiC31
  • Insert Size (bp)
    285

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13 -21
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gateway destination vector with phiC31 attB site, for Drosophila transformation into attP landing sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-attB was a gift from Jeff Sekelsky (Addgene plasmid # 30325 ; http://n2t.net/addgene:30325 ; RRID:Addgene_30325)
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