pEGFP ABR
(Plasmid
#30315)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 30315 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C1
- Backbone size w/o insert (bp) 4735
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZonula Occludens-1 actin-binding region
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Alt nameZO-1 ABR
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SpeciesH. sapiens (human)
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Insert Size (bp)723
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Mutationcontains only amino acids 1159-1382
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GenBank IDNP_003248.3
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Entrez GeneTJP1 (a.k.a. ZO-1)
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Tags
/ Fusion Proteins
- GFP (N terminal on backbone)
- vsvg (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Eco RI (not destroyed)
- 3′ cloning site Sal I (destroyed during cloning)
- 5′ sequencing primer pEGFP-N (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP ABR was a gift from Alan Fanning (Addgene plasmid # 30315 ; http://n2t.net/addgene:30315 ; RRID:Addgene_30315) -
For your References section:
Isolation and functional characterization of the actin binding region in the tight junction protein ZO-1. Fanning AS, Ma TY, Anderson JM. FASEB J. 2002 Nov . 16(13):1835-7. 10.1096/fj.02-0121fje PubMed 12354695