pmCherry-C1-RanQ69L
(Plasmid #30309)

• Depositing Lab: Jay Brenman
• Publication: Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-42.

Backbone

• Vector backbone: pmCherry-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RanQ69L
• Alt name: RAs-related Nuclear protein Q69L
• Alt name: Ran
• Species: N/A
• Insert Size (bp): 650
• Mutation: Glutamine 69 is changed to Leucine
• GenBank ID: NP_006316
• Entrez Gene: RAN (a.k.a. ARA24, Gsp1, TC4)
• Tag / Fusion Protein:
  • FLAG-Cherry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Andrew Wilde (University of Toronto)
• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry