pFLAG-Cherry-N1-PRKAG1
(Plasmid #30308)

• Depositing Lab: Jay Brenman
• Publication: Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-42.

Backbone

• Vector backbone: pCherry-FN1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AMP-activated Protein Kinase γ1
• Alt name: AMPKγ1
• Alt name: AMPKγ1
• Alt name: AMPK
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• GenBank ID: NP_002724
• Entrez Gene: PRKAA1 (a.k.a. AMPK, AMPK alpha 1, AMPKa1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mCherry (C terminal on backbone)
  • FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Reuben Shaw (UCSD)
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry