pEGFP-C1-PRKAA2ΔC
(Plasmid #30307)

• Depositing Lab: Jay Brenman
• Publication: Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-42.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AMP-activated Protein Kinase α2 ΔC
• Alt name: AMPKα2 ΔC
• Alt name: AMPKα2
• Alt name: AMPK
• Species: R. norvegicus (rat)
• Insert Size (bp): 1600
• Mutation: deleted amino acids Ser 527 to Arg 552
• GenBank ID: NP_076481
• Entrez Gene: Prkaa1 (a.k.a. AMPKalpha1)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: AddGene plasmid 15991

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry