Skip to main content
Addgene

pEGFP-C1-PRKAA1ΔC
(Plasmid #30306)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30306 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AMP-activated Protein Kinase α1 ΔC
  • Alt name
    AMPKα1ΔC
  • Alt name
    AMPKα1
  • Alt name
    AMPK
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1600
  • Mutation
    deleted amino acids Pro 526 to Gln 548
  • GenBank ID
    NP_001013385
  • Entrez Gene
    Prkaa1 (a.k.a. AMPKalpha1)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1-PRKAA1ΔC was a gift from Jay Brenman (Addgene plasmid # 30306 ; http://n2t.net/addgene:30306 ; RRID:Addgene_30306)

  • For your References section:

    Identification of a nuclear export signal in the catalytic subunit of AMP-activated protein kinase. Kazgan N, Williams T, Forsberg LJ, Brenman JE. Mol Biol Cell. 2010 Oct 1. 21(19):3433-42. 10.1091/mbc.E10-04-0347 PubMed 20685962
Related items:
Commonly requested with: