pET-3c-rhVTN-N
(Plasmid #30225)

• Depositing Lab: James Thomson
• Publication: Chen et al Nat Methods. 2011 Apr 10. ():.

Backbone

• Vector backbone: pET-3c
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4607
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5a in LB media
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: rhVitronectin with N terminus cleavage
• Alt name: VTN
• Alt name: rhVTN-N
• Alt name: VTN-N
• Species: H. sapiens (human)
• Insert Size (bp): 1254
• Mutation: deleted amino acids: 20*-62
• Entrez Gene: VTN (a.k.a. V75, VN, VNT)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NdeI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that the first 19 amino acids are the signal peptide for VTN, which is cleaved off on the mature protein during normally protein synthesis in mammalian cells. That means wild type mature VTN starts from aa20. That is why none of the four constructs have amino acid 1-19 and why pET-3c-rhVTN-N is a deletion of aa 20-62 from WT VTN.