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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 30223 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-3c
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 4607
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5a in LB media
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namerhVitronectin without deletions
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Alt nameVTN-FL
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Alt namerhVTN-FL
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Alt nameVTN
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SpeciesH. sapiens (human)
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Insert Size (bp)1380
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MutationNo deletions
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Entrez GeneVTN (a.k.a. V75, VN, VNT)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NdeI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note that the first 19 amino acids are the signal peptide for VTN, which is cleaved off on the mature protein during normally protein synthesis in mammalian cells. That means wild type mature VTN starts from aa20. None of the four constructs from this paper have amino acids 1-19.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-3c-rhVTN_FL was a gift from James Thomson (Addgene plasmid # 30223 ; http://n2t.net/addgene:30223 ; RRID:Addgene_30223) -
For your References section:
Chemically defined conditions for human iPSC derivation and culture. Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JM, Thomson JA. Nat Methods. 2011 Apr 10. ():. 10.1038/nmeth.1593 PubMed 21478862