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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 30217 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneAdenoX
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Backbone manufacturerClontech
- Backbone size (bp) 33000
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Vector typeAdenoviral
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer None
- 3′ sequencing primer None (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
He, A. and Pu, W. T. (2010) Genome-wide location analysis by pull down of in vivo biotinylated transcription factors. Curr Protoc Mol Biol Chapter 21, Unit 21.20
Please note that Addgene's sequencing results differ from the sequence provided by the depositing scientist at nucleotides 1778 and 1996. This mismatches are not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAd:TRE-gateway was a gift from William Pu (Addgene plasmid # 30217 ; http://n2t.net/addgene:30217 ; RRID:Addgene_30217) -
For your References section:
Co-occupancy by multiple cardiac transcription factors identifies transcriptional enhancers active in heart. He A, Kong SW, Ma Q, Pu WT. Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7. 10.1073/pnas.1016959108 PubMed 21415370