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Addgene

pENTR-flbio
(Plasmid #30216)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30216 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR3C
  • Backbone size w/o insert (bp) 2400
  • Vector type
    Gateway Entry plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    No gene
  • Insert Size (bp)
    2700
  • Tags / Fusion Proteins
    • Flag (C terminal on backbone)
    • Bio (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pENTR3c was re-engineered to allow fusion of flag-bio to the c-terminus of an ORF. The ORF is cloned in 5'-SalI-EcoRI-3'. The ccdb gene was removed. The fusion construct is transferred to a gateway destination vector by LR clonase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-flbio was a gift from William Pu (Addgene plasmid # 30216 ; http://n2t.net/addgene:30216 ; RRID:Addgene_30216)

  • For your References section:

    Co-occupancy by multiple cardiac transcription factors identifies transcriptional enhancers active in heart. He A, Kong SW, Ma Q, Pu WT. Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7. 10.1073/pnas.1016959108 PubMed 21415370
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