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pSG5-FER
(Plasmid #30191)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30191 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4076
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fer tyrosine kinase
  • Alt name
    FER
  • Alt name
    Fer (fps/fes related) tyrosine kinase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2950
  • GenBank ID
    NM_005246
  • Entrez Gene
    FER (a.k.a. PPP1R74, TYK3, p94-Fer)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fer pSG5. Fer is a non-receptor tyrosine kinase located on human chromosome 5q21. Gene ID 2241; HPRD 01491; NM_005246.

The construct was put together by Joanne ten Hoeve in 1993 and contains the complete coding region of human Fer.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5-FER was a gift from Nora Heisterkamp (Addgene plasmid # 30191 ; http://n2t.net/addgene:30191 ; RRID:Addgene_30191)

  • For your References section:

    Isolation and sequence analysis of a novel human tyrosine kinase gene. Hao QL, Heisterkamp N, Groffen J. Mol Cell Biol. 1989 Apr . 9(4):1587-93. PubMed 2725517
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