pBS-MK3
(Plasmid
#30190)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 30190 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript KS+
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2958
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMK-3
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Alt nameKcna3
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Alt nameKv1.3
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Alt nameKca1-3
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SpeciesM. musculus (mouse)
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Insert Size (bp)1746
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GenBank IDBC137667
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Entrez GeneKcna3 (a.k.a. Kca1-3, Kv1.3, Mk-3)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (destroyed during cloning)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-MK3 was a gift from Bruce Tempel (Addgene plasmid # 30190 ; http://n2t.net/addgene:30190 ; RRID:Addgene_30190)